Predictions of the model
# |
Genotype | Phenotype |
i. |
triple-cln triple-cln sic1∆ |
Inviable, G1 arrest (Wittenberg et al., 1990). Viable, large (Tyers, 1996). Inviable, G1 arrest (prediction). |
ii. |
cln3∆ bck2∆ cln3∆ bck2∆ sic1∆ |
Inviable, G1 arrest (Epstein & Cross, 1994) Inviable (Wijnen & Futcher, 1999). Inviable (prediction) |
iii. |
GAL-CLB5 sic1∆ |
Inviable, inefficient origin licensing Viable, size similar to WT (prediction). |
iv. |
CLB5-db∆ sic1∆ |
Inviable, inefficient origin licensing Viable, size similar to WT (prediction). |
v. |
GAL-CLB5-db∆ |
Inviable, inefficient origin licensing Viable, size similar to WT (prediction). Viable, size bigger than GAL-CLB5-db∆ 2XSIC1 (prediction). Still inviable (prediction). |
vi. |
CLB2-db∆ CLB2-db∆ GAL-SIC1 |
Arrests in telophase (Wasch & Cross, 2002). Viable (Cross, 2003). Viable (prediction). |
vii. |
cdc20∆ clb5∆ pds1∆ triple-deletion + 1.5X NET1
triple deletion + sic1∆ |
Viable (Shirayama et al., 1999). Arrests in telophase (prediction) Arrests in G1 (prediction). Viable (prediction). |
viii. |
cdc20∆ pds1∆ cdc20∆ pds1∆ TAB6-1 |
Arrests in telophase (Shirayama et al., 1999). Viable (prediction). Viable (prediction). |
ix. |
APC-A cdh1∆ APC-A sic1∆ |
Inviable in glucose (Cross, 2003). Viable (Cross, 2003). Viable (prediction). |
x. |
sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20 |
Viable (Archambault et al., 2003). Inviable, G2 arrest (prediction). |
xi. |
In nocodazole: net1-ts net1-ts 2X CLB2 (or 2X CLB5) net1-ts 2X CLN2 (or 2X CLN3) net1-ts sic1∆ (or cdc6∆2-49 or both) |
Loses viability (Visintin et al., 1999) Retains viability (prediction). Loses viability (prediction). Loses viability (prediction). |
xii. |
cln1∆ cln2∆ clb5∆ clb6∆ quadruple mutant + GAL-CLB2 |
Inviable, arrests in G1 (Schwob & Nasmyth, 1993). Able to do DNA synthesis and mitosis, but no bud formation. Becoming dinucleate, similar to cdc24-ts (prediction). |