Modeling the Budding Yeast Cell Cycle

List of simulated mutants

The phenotype of mutants in red do not coincide with the experiment data.


Cln mutants

  1. cln1∆ cln2∆
  2. GAL-CLN2 cln1∆ cln2∆
  3. cln1∆ cln2∆ sic1∆
  4. cln1∆ cln2∆ cdh1∆
  5. GAL-CLN2 cln1∆ cln2∆ cdh1∆
  6. cln3∆
  7. GAL-CLN3

Bck2 mutants

  1. bck2∆
  2. Multi-copy BCK2
  3. cln1∆ cln2∆ bck2∆
  4. cln3∆ bck2∆
  5. cln3∆ bck2∆ multi-copy CLN2
  6. cln3∆ bck2∆ GAL-CLN2 cln1∆ cln2∆
  7. cln3∆ bck2∆ sic1∆

cln1∆ cln2∆ cln3∆ strain

  1. cln1∆ cln2∆ cln3∆
  2. cln1∆ cln2∆ cln3∆ GAL-CLN2
  3. cln1∆ cln2∆ cln3∆ GAL-CLN3
  4. cln1∆ cln2∆ cln3∆ sic1∆
  5. cln1∆ cln2∆ cln3∆ cdh1∆
  6. cln1∆ cln2∆ cln3∆ multi-copy CLB5
  7. cln1∆ cln2∆ cln3∆ GAL-CLB5
  8. cln1∆ cln2∆ cln3∆ multi-copy BCK2
  9. cln1∆ cln2∆ cln3∆ GAL-CLB2
  10. cln1∆ cln2∆ cln3∆ apcts

Cdh1, Sic1 and Cdc6 mutants

  1. sic1∆
  2. GAL-SIC1
  3. GAL-SIC1-db∆
  4. GAL-SIC1 cln1∆ cln2∆
  5. GAL-SIC1 GAL-CLN2 cln1∆ cln2∆
  6. GAL-SIC1 cln1∆ cln2∆ cdh1∆
  7. GAL-SIC1 GAL-CLN2 cln1∆ cln2∆ cdh1∆
  8. cdh1∆
  9. Cdh1 constitutively active
  10. sic1∆ cdh1∆
  11. sic1∆ cdh1∆ GALL-CDC20
  12. cdc6∆2-49
  13. sic1∆ cdc6∆2-49
  14. cdh1∆ cdc6∆2-49
  15. sic1∆ cdc6∆2-49 cdh1∆
  16. sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
  17. swi5∆
  18. swi5∆ GAL-CLB2
  19. swi5∆ cdh1∆
  20. swi5∆ cdh1∆ GAL-SIC1

Clb1 Clb2 mutants

  1. clb1∆ clb2∆
  2. CLB1 clb2∆
  3. GAL-CLB2
  4. Multicopy GAL-CLB2
  5. CLB1 clb2∆ cdh1∆
  6. CLB1 clb2∆ pds1∆
  7. GAL-CLB2 sic1∆
  8. GAL-CLB2 cdh1∆
  9. CLB2-db∆
  10. CLB2-db∆ in galactose
  11. CLB2-db∆ multi-copy SIC1
  12. CLB2-db∆ GAL-SIC1
  13. CLB2-db∆ multi-copy CDC6
  14. CLB2-db∆ cllb5∆
  15. CLB2-db∆ cllb5∆ in galactose
  16. GAL-CLB2-db∆

Clb5 Clb6 mutants

  1. cllb5∆ clb6∆
  2. cln1∆ cln2∆ cllb5∆ clb6∆
  3. GAL-CLB5
  4. GAL-CLB5 sic1∆
  5. GAL-CLB5 cdh1∆
  6. CLB5-db∆
  7. CLB5-db∆ sic1∆
  8. CLB5-db∆ pds1∆
  9. CLB5-db∆ pds1∆ cdc20∆
  10. GAL-CLB5-db∆

Cdc20 mutants

  1. cdc20ts
  2. cdc20∆ cllb5∆
  3. cdc20∆ pds1∆
  4. cdc20∆ pds1∆ cllb5∆
  5. GAL-CDC20
  6. cdc20ts mad2∆
  7. cdc20ts bub2∆

Pds1/Esp1 interaction

  1. pds1∆
  2. esp1ts
  3. PDS1-db∆
  4. GAL-PDS1-db∆
  5. GAL-PDS1-db∆ esp1ts
  6. GAL-ESP1 cdc20ts

MEN pathway mutants

  1. tem1∆
  2. GAL-TEM1
  3. tem1ts multi-copy CDC15
  4. tem1ts GAL-CDC15
  5. tem1∆ net1ts
  6. tem1∆ multi-copy CDC14
  7. cdc15∆
  8. Multi-copy CDC15
  9. cdc15ts multi-copy TEM1
  10. cdc15∆ net1ts
  11. cdc15ts multi-copy CDC14

Exit-of-mitosis mutants

  1. net1ts
  2. GAL-NET1
  3. cdc14ts
  4. GAL-CDC14
  5. GAL-NET1 GAL-CDC14
  6. net1ts cdc20ts
  7. cdc14ts GAL-SIC1
  8. cdc14ts then GAL-SIC1
  9. cdc14ts sic1∆ at permissive temperature
  10. cdc14ts cdh1∆ at permissive temperature
  11. cdc14ts GAL-CLN2 at permissive temperature
  12. TAB6-1
  13. TAB6-1 cdc15ts
  14. TAB6-1 cllb5∆ clb6∆
  15. TAB6-1 CLB1 clb2∆

Checkpoint mutants

  1. mad2∆
  2. bub2∆
  3. mad2∆ bub2∆
  4. WT in nocodazole
  5. mad2∆ in nocodazole
  6. mad2∆ GAL-TEM1 in nocodazole
  7. mad2∆ pds1∆ in nocodazole
  8. bub2∆ in nocodazole
  9. bub2∆ pds1∆ in nocodazole
  10. bub2∆ mad2∆ in nocodazole
  11. pds1∆ in nocodazole
  12. net1ts in nocodazole

APC mutants

  1. APC-A
  2. APC-A cdh1∆
  3. APC-A cdh1∆ in galactose
  4. APC-A cdh1∆ multi-copy SIC1
  5. APC-A cdh1∆ GAL-SIC1
  6. APC-A cdh1∆ multi-copy CDC6
  7. APC-A cdh1∆ GAL-CDC6
  8. APC-A cdh1∆ multi-copy CDC20
  9. APC-A sic1∆
  10. APC-A GAL-CLB2